The United captain missed the Stoke City game on Tuesday night due to a knee injury picked up late on against Hull City last weekend but Rooney will return to the line-up for the crunch match against Southampton.
The Saints sit just above United in the league and a win at St. Mary’s will move United into 3rd place and keep them on the heels of Manchester City.
Ronald Koeman’s men have lost their last two games and will be missing Morgan Schneiderlin, Jack Cork and possibly Toby Alderweireled on Monday.
Rooney has been in top form lately, scoring three goals over the international break before returning to Old Trafford and scoring the crucial second goal away at Arsenal before adding to his tally against Hull on Saturday.
With Robin van Persie still not hitting top form and Radamel Falcao slowly returning to full fitness, Rooney’s return is a boost ahead of two big games leading into the Christmas period.