Rooney and Martial missed the game against Watford last weekend due to illness and injury, respectively.
Fellaini, meanwhile, had been sidelined for a number of weeks due to an unspecified knock. Van Gaal revealed that the trio will be involved against PSV after taking part in training on Tuesday.
“Fellaini, Martial and Rooney have shown on the training ground their fitness. Every match it’s the same procedure,” van Gaal said.
The return of Martial and Rooney, in particular, will be a boost for van Gaal as he prepares for the clash against PSV.
Memphis Depay played up top against Watford and impressed, scoring United’s opener, but van Gaal will be keen to have as many options as possible available to him. United can secure qualification to the next round of the Champions League with a win against the Dutch side and they will be confident of doing so with Rooney and Martial back in the squad.